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SNuPE Protocol
關鍵詞： snupe protocol來源： 互聯網
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Make sure that there is enough dCTP and TTP (or dGTP and dATP) in the hot room Set up your PCR master mix.? Remember that you need to do a reaction for dCTP and TTP for each sample 10xPCR Buffer? 2.5uL 0.1M MgCl2? 0.375ul Primer? 1ul Taq? 1ul dH20? - enough to bring final volume to 25 ul
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