Assay Design for MIP Genotyping

Description: For each SNP a single MIP probe is designed. A minimum of 5000 probes are designed as a probe set. 1) MIP probe design Two homologies are designed that abut, but do not overlap the SNP. The tag sequence, two primer sequences and a restriction sequence are added to homology 1 and homology 2 in the order (5' - 3') homology 2, tag sequence, restriction site, primer P2, primer P1, and homology 1.

?

上一篇：以食為天--水稻基因組計劃 ? 下一篇：HapMap assay-design protocols