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Brandford法測定蛋白濃度
關鍵詞： Brandford法 蛋白濃度來源： 互聯網
Bradford quantification of protein concentration Brandford法測定蛋白濃度
Standard Curve
| m g of standard BSA | 0.2 mg/mL BSA stock(m L) | dH2 O (m L) | Bradford mix (m L) |
1 | 5 | 795 | 200 |
2 | 10 | 790 | 200 |
4 | 20 | 780 | 200 |
6 | 30 | 770 | 200 |
8 | 40 | 760 | 200 |
Wait for 5 minutes and then read absorbance at 595nm
At the same time,prepare your samples of interest as above and meaure absorbance after measuring the standards.Take an average of the standard sample and determine the absorbance that defines 1 mg of BSA and use that to measure the concentration of the unknown samples.
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