細菌人工染色體

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• The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript) (Clemson University Genomics Institute) ??
• Construction of BAC Library (Caltech) Guideline for BAC library preparation, the followings are described in great detail?
  • Preparation of BAC vector DNA
  • Preparation of source DNA
  • Ligation and Electroporation
  • Preparation of BAC DNA ???
• BAC Manual (Texas A&M BAC Center) Provide details in the theory and practice for the construction, characterization, and screening of BAC libraries.
  • Introduction
  • MegaBase DNA Preparation
  • BAC Vector Preparation
  • Solutions ??
• Hybridization of High Density Arrayed BAC Nylon Filter Blots (Caltech) Procedures for isolation and purification of cDNA inserts using polymerase chain reaction, probe labeling, probe hybridization and more... ???
• BAC/PAC/Fosmid Prep (WUGSC) ???
• BAC Sequencing with ET primers (WUGSC) ??
• BAC End-Sequencing (Caltech) Prepare BAC DNA for sequencing ??
• Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation (Roe Lab)

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