毛細柱安裝快捷指南
Precolumn installation check list
預檢查清單
1. Replace oxygen, moisture, and hydrocarbon traps as
needed.
如有必要，替換掉除氧、除濕、除烴的捕集阱。
2. Check gas cylinder pressure to ensure that an adequate
supply of carrier, makeup, and fuel gases is available. Minimum carrier gas
purity percentages: helium 99.9995, hydrogen 99.9995.
檢查氣瓶壓力，確認載氣、補充氣、可燃氣合適。載氣純度百分比最小應達到：氦氣，99.995;氫氣，99.995。
3. Clean the injector port, replace critical injection port
seals, replace injection port liners, and change septa as needed.
清洗進樣口，如有必要，更換密封墊，襯管，隔墊。
4. Check detector seals, and replace as necessary. Clean or
replace detector jets as necessary.
檢查檢測器密封墊，如有必要就替換。如有必要清洗或替換檢測器噴嘴。
5. Carefully inspect the column for damage or
breakage.
仔細檢查柱是否破損。
6. Gather the necessary installation tools: you will need a
column cutter, column nuts, ferrules, a magnifying loop, and typewriter
correction fluid.
收集必要的安裝工具：柱切割器、柱螺母、墊圈、放大鏡、打字機改正液。
Installing the column
安裝柱
1. Uncoil approximately 0.5m of tubing from the column basket
at both ends of the column for injector and detector installation. Avoid sharp
bends in the tubing.
從柱圈兩端各解開約 0.5米的柱，以便進樣口和檢測器安裝。
2. Mount the column in the oven. Use the hanging bracket if
available.
在柱箱中裝柱。如果可能的話，用柱懸掛架。
3. Install the column nut and Vespel or graphite ferrule at
each column end; pull the nut and ferrule down the tubing approximately 5
cm.
在柱兩端按裝螺母和Vespel或石墨墊。將螺母和墊圈順著柱拉下約5厘米。
4. Score the column. Use a light touch to score the column
about 4 to 5 cm from each end.
切割柱。距柱每個尾端約4-5cm處輕劃一下。
5. Make a clean break. Grasp the column between the thumb and
forefinger as close to the score point as possible. Gently pull and bend the
column. The column should part easily. If the column doesn’t break easily, don’t
force it! Score the column again in different place, and try for a clean
break.
確保整齊的劃口。用拇指和食指抓住柱，離劃痕處越近越好。輕輕地彎曲柱。柱應該很容易地折斷。如果柱不能折斷，不能強行用力。在不同的地方再割一次柱，嘗試獲是整齊地劃口。
6. Use a magnifying loop to inspect the cut. Make sure the cut
is square across the tubing with no polyimide or glass fragments at the end of
the tube.
用放大鏡檢查劃口。確認劃口正交于柱，柱端沒有聚酰亞胺或石英碎片。
7. Install the column in the inlet. Check the GC
manufacturer’s instrument manual for the correct insertion distance. Mark the
correct distance on the column with typewriter correction fluid. Insert the
column into the injector. Finger tighten the column nut until it starts to grab
the column, and then tighten the nut an additional 1/4 to 1/2 turn so that the
column can’t be pulled from the fitting when gentle pressure is
applied.
在進樣口裝柱。查閱GC生產商的儀器說明書，以便獲取正確的安裝距離。用打字機改正液在柱子上標識正確地距離。將柱插入進樣口。用手擰緊柱螺母直至其開始夾住柱子。再擰1/4-1/2圈，這樣輕輕拉動時，柱子不會脫出來。
8. Turn on the carrier gas, and establish the proper flow
rate. Set head pressure, split flow, and septum purge flow to appropriate
levels. See Table B for nominal head pressure. If using a split/splitless inlet,
check that the purge valve is ON.
接通載氣，調至合適地流速。設置相應的柱頭壓、分流比、隔墊吹掃。常規地柱頭壓參考表B。如果使用分流/不分流進樣口，檢查分流閥是否打開。
9. Confirm carrier gas flow through the column. Immerse the
end of the column in a vial of acetone and check bubbles.
確保載氣通過了柱子。將柱尾端入丙酮中檢查氣泡。
10. Install the column into detector. Check the instrument
maufacture’s manual for the proper insertion distance.
將柱子裝進檢測器。查閱儀器生產商的說明書，以便獲取合適的安裝距離。
11. Check for leaks. This if very important. Don’t enven think
about heating the column without thoroughly checking for leaks.
檢漏。這非常重要。不能不檢漏就加熱柱子。
12. Establish proper injector and detector
temprature.
給進樣口和檢測器設定恰當的溫度。
13. Establish proper makeup and detector gas flows. Ignite or
turn on the detector.
設定恰當的補充氣和檢測氣流量。點火或打開檢測器。
14. Purge the column for a minimum of 10 minutes at ambient
temperature(approximately six column volumes).
在室溫下冷吹柱子至少10分鐘(約6倍的柱體積)。
15. Inject a nonretained substance to check for proper
injector installation. Examples: butane or methane(FID),headspace vapors from
acetonitrile(NPD), methylene chloride(ECD),air(TCD),argon(mass spectrometer).
Proper installation is indicated by a symmetrical peak. If tailing is observed,
repeat the injector installation process.
進沒有保留的物質檢查進樣口安裝是否合適。如丁烷或甲烷
(FID)，乙腈頂空蒸汽(NPD)，氯仿(ECD)，空氣(TCD)，氬氣(MSD)。安裝合適會得到對稱的峰形。如果峰拖尾，重新安裝進樣口。
