This European Standard specifies a performance test of loaded collection substrates for samplers for the inhalable, thoracic or respirable aerosol fractions and, as alternative, a handling test, both for testing transport losses of aerosol sampler substrates under prescribed conditions in order to calculate the expanded uncertainty of a measuring procedure according to EN 13205-1:2014, Annex A. The transport test involves shipping loaded substrates with ordinary mail, whereas the handling test uses a shaker. This part of EN 13205 applies to all samplers used for the health-related sampling of particles in workplace air.