熱門搜索詞：
Biotinylation of Antibody
關鍵詞： biotinylation of antibody來源： 互聯網
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Materials
- 0.1M NaHC03 pH9
- DMSO
- NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759)
- PBS
Procedure
Dialyze the sample against carbonate buffer. After dialysis, adjust sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room temperature or overnight at 4C. Dialyze against PBS.
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