Multilocus sequence typing (MLST) is a genotyping method that is well suited for studying the population genetics and evolution of Staphylococcus epidermidis . The central MLST database for S. epidermidis continues to grow, and new analysis methods for extracting historical information from MLST data continue to be developed. Even in this era of whole-genome sequencing, MLST provides a reference genotyping method, and the central MLST database provides a unique catalog of genetic variants.