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• Gram Staining (+\-) (William H. Heidcamp) ??
• Gram-Staining Procedure (MEDIC, U of Texas) Very nice and detailed method description for Gram staining ??
• Acid-Fast Stain (Putt's Method) To detect the presence of acid-fast mycobacteria in tissue sections
• Smear Preparation (MEDIC, U of Texas) The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium. ??
• Alkaline Phosphatase and Phospholipase C Assay (Hancock Lab) (Accessible only by Internet Explorer) ??
• Bacteria Agglutination Assay (Hancock Lab) (Accessible only by Internet Explorer) Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120o C for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cells grown to mid log phase, then centrifuged and resuspended in saline. ???
• Carbohydrate Assay (Hancock Lab) (Accessible only by Internet Explorer) This assay is used to determine the relative amounts of LPS CHO present in a given strain and can be done on one set of samples and then scanned at the usr/localious wavelengths for reasonable data on the 3 types of sugars. ??
• Dintrophenylation Assay for Polymixin B (Hancock Lab) (Accessible only by Internet Explorer) ??
• Endotoxin Assay (Hancock Lab) (Accessible only by Internet Explorer) This assay measures the shift in the absorbance maximum of the dye due to binding to endotoxin ??
• Indirect Immunfluoresence ?(Hancock Lab) (Accessible only by Internet Explorer) ??
• Liposome Swelling Assay (Hancock Lab) (Accessible only by Internet Explorer) Also including method for liposome Isolation and? liposome preparatio n ??
• Preparation of E Coli Protein for SDS-PAGE (Hahn Lab) Simplified method for analyzing E coli protein

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