從入門到精通，SFC于醫藥工業之應用

超臨界流體色譜技術 (Supercritical fluid chromatography, SFC) ，顧名思義，即流動相既不是氣體也不是液體，而是使用超過臨界溫度和臨界壓力下的單一態流體(Supercritical Fluid)作流動相進行分離分析、制備純化的色譜技術。

SFC的原理與液相色譜的原理類似，然而利用超臨界流體（通常為二氧化碳CO₂）取代有機溶劑或水作為主要流動相，從本質上來說是一種高效的正相色譜技術。作為流動相所使用的超臨界流體CO₂具有獨特的低粘度和高擴散性能而成為一種智能溶劑，因具有與液體相近的密度，對各種物質有較強的溶解性，難揮發性和熱穩定性差的物質分析均適用；同時，超臨界流體的粘度近于氣體，比液體約低1～２個數量級，而它的擴散系數在氣體和液體之間，因而具有較快的傳質速度和較小的傳質阻力，分離過程中峰形變窄、柱效增加、分析速度加快。由此可見，SFC揚HPLC與GC之長、避GC及HPLC之短，具有獨特的優越性，應用前景極為廣闊。

由于SFC采用天然“綠色”的超臨界流體CO₂作為溶劑，無需有機溶劑回收處理，省事經濟環保，節能降耗又可持續使用，因而SFC對生物醫藥、精細化工、生態環境、農業食品等行業具有獨特的吸引力。

SFC在制藥行業，尤其是手性藥物開發領域，由于其能夠有效地利用液相色譜中常用的手性固定相 (chiral stationary phase，CSP) 且與GC、HPLC體系中使用的檢測器完全兼容， SFC是一種理想的手性分離方法。因為它運行速度更快，溶劑用量更少，樣品后處理更簡單，總體上來講，SFC是一種更經濟、更綠色的方法，在手性化合物分離方面性能卓越，大有超越高效液相色譜法（HPLC）之趨勢，為分離純化和手性制備分離的有益替代方案。

Ps：從入門到精通，SFC于醫藥工業之應用學習資料

2007年

J. David Pinkston, The Procter & Gamble Company   寶潔公司

SFC for Fun and Profit: 45 Years of Promise, Progress, and Phrustration

http://www.greenchemistrygroup.org/pdf/sciprog2007/2_david_pinkston.pdf

Geoffrey B Cox, Chiral Technologies, Inc

Solvent and stationary phase selectivity in enantioselective SFC

http://www.greenchemistrygroup.org/pdf/sciprog2007/11_geoffrey_cox.pdf

Dauh-Rurng Wu, Bristol-Myers Squibb Company   百時美施貴寶

Chiral Supercritical Fluid Chromatography in Drug Discovery: from Analytical to Multigram-Scale

http://www.greenchemistrygroup.org/pdf/sciprog2007/3_dauh-rurng_wu.pdf

Larry Miller, Matt Potter, Wes Barnhart, Kyung Gahm and John Eschelbach, Amgen   安進

Application of SFC in Pharmaceutical Discovery and Early Development

www.greenchemistrygroup.org/pdf/sciprog2007/08_larry_miller.pdf

Ling S. Xiao, Xinxin Han, James B. Murphy and Mary Gasper, Pfizer Global Research & Development   輝瑞

A Comparative Study of Fast Orthogonal Chiral Screening Methods by SFC and Normal Phase HPLC

http://www.greenchemistrygroup.org/pdf/sciprog2007/james_murphy_pfizer.pdf

2008年

Insights into chiral recognition mechanisms on varied stationary phases in supercritical fluid chromatography

http://www.univ-orleans.fr/icoa/communications/com2008/west.pdf

Larry Miller, Matt Potter and Ian Sebastian, Amgen, USA   安進

Evaluation of Operating Conditions in Analytical and Preparative SFC

http://www.greenchemistrygroup.org/pdf/sciprog2008/MI1745Miller.pdf

Fiona J Bell, AstraZeneca   阿斯利康

SFC At AstraZeneca Alderley Park

http://www.greenchemistrygroup.org/pdf/sciprog2008/Bell_Second_International_Conference_on_Packed-Column_SFC,_Switzerland,_Oct_1st-Oct_2nd_2008A0[1].pdf

Preparative Chiral Chromatography in Pharmaceutical R&D   安進

www.chiraltech.com/enews/MilleCT07pres.pdf

2009年

Christine Aurigemma, Pfizer   輝瑞

Breaking the Flow Rate Barrier: Utilization of High Flow Mass-Directed SFC for Pharmaceutical Applications

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Christine%20Aurigemma-Pfizer.pdf

Georg Diehl, Novartis   諾華

SFC-A Versatile Tool in the Analysis and Purification of Pharmaceutical Compounds

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Georg%20Diehl-Novartis.pdf

Mark Hayward, Lundbeck   丹麥靈北

SFC with Tandem Mass Spectrometry to Evaluate the in vivo Disposition of Individual Stereoisomers of Drugs

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Hayward-Lundbeck.pdf

Chiral Separations using SFC: Applications from Discovery through Development

http://sfc-ms.com/pdfs/webinars/chiral_sep_applic.pdf

A Screening Process for Development of Enantioselective Supercritical Fluid Chromatography Separation Methods

http://www.chiraltech.com/app/LCGC_MethDev.pdf

Dauh-Rurng Wu, Bristol Myers Squibb   百時美施貴寶

Preparative Chiral SFC in Drug Discovery: Promises and challenges

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Dauh-Rurng%20Wu-BMS.pdf

Larry Miller, Amgen   安進

Evaluation of Non-Traditional Co-Solvents for Routine Pharma Discovery Support

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Larry%20Miller-Amgen.pdf

Zhenyu Wang, Schering-Plough   先靈葆雅

SFC-a Powerful Tool for Polymethoxyflavone Isolation and Analysis

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Zhenyu%20Wang-Schering-Plough.pdf

聲明：上述SFC相關資料，來自 2007、2008、2009 SFC International Conference，僅供學習參考，勿作它用，謝謝！