商品化的手性色譜柱介紹(二)

Chiral Analytical HPLC Columns

Eka Chemicals offer two chiral phases: Kromasil CHI-DMB & CHI-TBB, in which acylated N,N'-diallyl-l-tartardiamide network polymers have been covalently bound to Kromasil.

Kromasil CHI-DMB
Chiral Polymer:	0,0'-bis (3,5-dimethylbenzoyl)-N,N'-diallyl-L-tartardiamide
Particle Sizes:	5μm & 10μm
Surface Area:	340m2/g
Pore Size:	100?
Carbon Load:	14.5%
Nitrogen Load:	0.6%

Kromasil CHI-TBB
Chiral Polymer:	0,0'-bis (4-tert-butylbenzoyl)-N,N'-diallyl-L-tartardiamide
Particle Sizes:	5μm & 10μm
Surface Area:	340m2/g
Pore Size:	100?
Carbon Load:	15.0%
Nitrogen Load:	0.6%

Two key steps are involved in their synthesis. The chiral monomers are reacted with a multifunctional hydrosilane yielding a network polymer incorporating a bifunctional C2-symmetric chiral selector. The chiral polymer is covalently bonded to functionalised Kromasil silica.

Description	Quote Part No.
Kromasil 5μm CHI-DMB 250 x 4.6mm	KR100-5CHI-DMB-250A
Kromasil 5μm CHI-TBB 250 x 4.6mm	KR100-5CHI-TBB-250A
Kromasil 10μm CHI-DMB 250 x 4.6mm	KR100-10CHI-DMB-250A
Kromasil 10μm CHI-TBB 250 x 4.6mm	KR100-10CHI-TBB-250A

ChromTech - Chiral Analytical HPLC Columns

ChromTech formally of Sweden, now based in the UK, manufacture 3 specific phases for the HPLC separation of chiral compounds.

ChromTech Chiral Phases:

• spherical porous silica
• reversed-phase applications
• unique separation characteristics
• 150+ cited references
• over 200 chiral molecules resolved

Phase	Chiral selector	Particle size (μm)	Application
CHIRAL-AGP	α1-acid glycoprotein	5	broad: amines; acids; alcohols; esters; sulphoxides
CHIRAL-CBH	cellobiohydrolase	5	alcohols; phenols; carbonyls; amides; ethers; esters
CHIRAL-HSA	human serum albumin	5	weak acids; strong acids; zwitterionic compounds

Method development:

Solution pH, organic solvent modifier & aqueous buffer affect the performance of the columns.

Parameter	Typical conditions	Effect of varying parameter
pH	4 - 7	variable
buffer	phosphate 0.01 - 0.1M	increase in buffer concentration can increase retension & enantioselectivity
organic solvent	propan-2-ol, acetonitrile,methanol 5-15%	Selection of solvent strongly affects enantioselectivity. Higher organic solvent ratios reduce retension time for all phases. For both AGP & HSA columns, enantioselectivity is simultaneously reduced, whilst for CBH columns it is often increased.

Ordering Information:

Semi prep columns available without discount

Phase	Column dimensions
	100x2	150x2	100x3	150x3	50x4	100x4	150x4
CHIRAL-AGP	AGP100.2	AGP150.2	AGP100.3	AGP150.3	AGP50.4	AGP100.4	AGP150.4
CHIRAL-CBH	CBH100.2	CBH150.2	CBH100.3	CBH150.3	CBH50.4	CBH100.4	CBH150.4
CHIRAL-HSA	HSA100.2	HSA150.2	HSA100.3	HSA150.3	HSA50.4	HSA100.4	HSA150.4

Regis Technologies - Chiral Analytical HPLC Columns

DACH-DNB chiral stationary phase

The DACH-DNB CSP has the following advantages:

• column durability due to covalent bonding
• ability to invert elution order
• excellent chromatographic efficiency
• universal solvent compatability

Cat #	Description
788101	(R,R)-DACH-DNB 25cm x 4.6mm 5μm 100?
788102	(R,R)-DACH-DNB 25cm x 10.0mm 5μm 100?
788201	(S,S)-DACH-DNB 25cm x 4.6mm 5μm 100?
788202	(S,S)-DACH-DNB 25cm x 10.0mm 5μm 100?

The DACH-DNB material is also available for large-scale preparative separation

Other analytical chiral columns available from Regis

Cat #	Description
786201	(R,R)-Whelk-O 1 25cm x 4.6mm 5μm 100?
786101	(S,S)-Whelk-O 1 25cm x 4.6mm 5μm 100?
786515	(R,R)-Whelk-O 1 25cm x 4.6mm 10μm 100?
786615	(S,S)-Whelk-O 1 25cm x 4.6mm 10μm 100?
786315	(R,R)-Whelk-O 2 25cm x 4.6mm 10μm 100?
786415	(S,S)-Whelk-O 2 25cm x 4.6mm 10μm 100?
787200	(R,R)-ULMO 25cm x 4.6mm 5μm 100?
787100	(S,S)-ULMO 25cm x 4.6mm 5μm 100?
731044	(3R,4S)-Pirkle 1-J 25cm x 4.6mm 5μm 100?
731045	(3S,4R)-Pirkle 1-J 25cm x 4.6mm 5μm 100?
731043	(R,R)-?-Gem 1 25cm x 4.6mm 5μm 100?
781029	(S,S)-?-Gem 1 25cm x 4.6mm 5μm 100?
785035	(R,R)-α-Burke 2 25cm x 4.6mm 5μm 100?
735037	(S,S)-α-Burke 2 25cm x 4.6mm 5μm 100?
731054	D-Leucine 25cm x 4.6mm 5μm 100?
731041	L-Leucine 25cm x 4.6mm 5μm 100?
731021	D-Phenylglycine 25cm x 4.6mm 5μm 100?
731024	L-Phenylglycine 25cm x 4.6mm 5μm 100?
731034	L-Naphthylleucine 25cm x 4.6mm 5μm 100?

 

來源: http://www.hichrom.co.uk/technical/chiral.htm 

 

The Regis Pirkle-Concept Chiral HPLC Column Its Nature, Care, and Use

http://www.registech.com/chiral/care_and_use.html

http://www.registech.com/chiral/