現在做蛋白組學最牛的質譜是……
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現在做蛋白組學最牛的質譜是什么？
幾年前的答案是：Q-TOF或者FT
現在呢？
當然是Orbitrap了！
Thermo 的Orbitrap在國內好像有幾臺了，近期可能還要增加，呵呵，很貴的說。
發篇Anal. Chem.最新的封面文章，PDF的全文在附件里：）
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ancham_big_051507.jpg
Anal. Chem., 79 (10), 3525 -3534, 2007. 10.1021/ac070020k S0003-2700(07)00020-0
Web Release Date: April 19, 2007
Copyright ? 2007 American Chemical Society
Implementation of Electron-Transfer Dissociation on a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer
Graeme C. McAlister, Doug Phanstiel, David M. Good, W. Travis Berggren, and Joshua J. Coon*
Departments of Chemistry and Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706, and WiCell Research Institute, Madison, Wisconsin 53706
Received for review January 3, 2007. Accepted March 20, 2007.
Abstract:
We describe the adaptation of a hybrid quadrupole linear ion trap-orbitrap mass spectrometer to accommodate electron-transfer ion/ion reactions (ETD) for peptide and protein characterization. The method utilizes pulsed, dual electrospray ion sources and requires minimal instrument modification. Switching between cation and reagent anion injection schemes is automated and accomplished within a few hundred milliseconds. Ion/ion reactions are conducted within the linear ion trap, after which the c- and z-type product ions are passed to the orbitrap for high-resolution m/z analysis. With this arrangement, mass accuracies are typically measured to within 2 ppm at a resolving power of ~60 000. Using large peptides and intact proteins, we demonstrate such capabilities will accelerate our ability to interrogate high-mass species. To illustrate compatibility with automated data-dependent analysis and subsequent data processing, we couple the technique with an online chromatographic separation of a yeast whole-cell lysate followed by peptide identification using ProSight PC. Fairly long pulsing times and relatively low ET efficiency, as compared to conventional ETD instrumentation, are the main drawbacks of this approach. Still, our results suggest that the implementation of ETD on sensitive, high-resolution, and high-mass accuracy hybrid instrumentation, such as the orbitrap, will substantially propel the emergent fields of middle- and top-down proteomics.
幾年前的答案是：Q-TOF或者FT
現在呢？
當然是Orbitrap了！
Thermo 的Orbitrap在國內好像有幾臺了，近期可能還要增加，呵呵，很貴的說。
發篇Anal. Chem.最新的封面文章，PDF的全文在附件里：）
——————————————————————————————————————
ancham_big_051507.jpg
Anal. Chem., 79 (10), 3525 -3534, 2007. 10.1021/ac070020k S0003-2700(07)00020-0
Web Release Date: April 19, 2007
Copyright ? 2007 American Chemical Society
Implementation of Electron-Transfer Dissociation on a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer
Graeme C. McAlister, Doug Phanstiel, David M. Good, W. Travis Berggren, and Joshua J. Coon*
Departments of Chemistry and Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706, and WiCell Research Institute, Madison, Wisconsin 53706
Received for review January 3, 2007. Accepted March 20, 2007.
Abstract:
We describe the adaptation of a hybrid quadrupole linear ion trap-orbitrap mass spectrometer to accommodate electron-transfer ion/ion reactions (ETD) for peptide and protein characterization. The method utilizes pulsed, dual electrospray ion sources and requires minimal instrument modification. Switching between cation and reagent anion injection schemes is automated and accomplished within a few hundred milliseconds. Ion/ion reactions are conducted within the linear ion trap, after which the c- and z-type product ions are passed to the orbitrap for high-resolution m/z analysis. With this arrangement, mass accuracies are typically measured to within 2 ppm at a resolving power of ~60 000. Using large peptides and intact proteins, we demonstrate such capabilities will accelerate our ability to interrogate high-mass species. To illustrate compatibility with automated data-dependent analysis and subsequent data processing, we couple the technique with an online chromatographic separation of a yeast whole-cell lysate followed by peptide identification using ProSight PC. Fairly long pulsing times and relatively low ET efficiency, as compared to conventional ETD instrumentation, are the main drawbacks of this approach. Still, our results suggest that the implementation of ETD on sensitive, high-resolution, and high-mass accuracy hybrid instrumentation, such as the orbitrap, will substantially propel the emergent fields of middle- and top-down proteomics.
ac070020k.rar
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dingdang發布于2007-07-18 01:09:00
- 嚴重同意！
- dingdang發布于2007-07-18 01:09:48
- 中國現在的Orbitrap：中科院生化所2套，復旦大學，浙江大學。
中國的LTQ FT：軍事醫學科學院，醫科院藥物所
景仰啊！
longcz發布于2007-07-18 01:10:17
- 但是中國現在蛋白組學做得好的單位基本沒有，比如就沒有在Nature, Science,Cell上發過文章的。
lily發布于2007-07-18 01:11:59
- Obitrap太貴了，如果沒錢怎么做啊？
haha發布于2007-07-18 01:15:27
- 好消息：聽說現在Orbitrap也有一種比較便宜的了。
- dingdang發布于2007-07-18 01:16:05
- Orbitrap現在有了兩款，一個是主要用于小分子的Orbitrap Discovery，一個是Orbitrap XL，后者用于蛋白質組學。
其實，我比較贊同大家不要太依賴于目前市面上最先進的儀器，因為如果把賭注押到這上面來的話，一是新技術儀器剛推出時非常昂貴。二是新技術需要人們不斷去適應。如果大家都還沒有，自己會花很多時間去適應，而且技術不是特別穩定時還有不成熟的問題。
所以，買一個國外大家用過都反映不錯，在新款推出時，買一個老款是比較劃算的。
我就見過最初買AB TOF/TOF 4700花60多萬美金的，但后來4800出來后，買4700就只花30多萬美金。
反正，最終是靠人。不是靠儀器。
- longcz發布于2007-07-18 01:16:56
- 同意樓上的觀點，總有新的儀器出來啊，不能見一個買一個，哈哈
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