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SARS-CoV-2 (2019-nCoV) Spike Protein 蛋白現貨(S1 Subunit, 10 x His Tag)

上一篇 / 下一篇 2020-03-26 09:51:19/ 個人分類：抗體

Cat. Number	E007
Chemical Name	SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, 10 x His Tag) TEL 13917250134
CAS Number	E007
Qty 1	1mg
Qty 2	10mg
Synonym	NCP-CoV Spike Protein, 2019-nCoV
Storage condition	-20℃ to -80℃
References	SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag): 產品信息純度 > 90 % as determined by SDS-PAGE. 蛋白質序列 : VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARAHHHHHHHHHH 內毒素 < 1.0 EU per μg protein as determined by the LAL method. 生物活性 The 2019-nCoV Spike Protein (S1 Subunit, His Tag) can bind with Human ACE2 in functional ELISA assay. 蛋白構建 A DNA sequence encoding the NCP-CoV(2019-nCoV) spike protein S1 Subunit was expressed with a polyhistidine tag at the C-terminus. 表達宿主 HEK293 Cells 種屬 2019-nCoV 預測 N 端 Val 分子量 The recombinant NCP-CoV(2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa. 緩沖液 Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. 運輸方式 In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. 穩定性 & 儲存條件 Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. 復溶 A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag): 別稱 coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; ncov RBD Protein, 2019-nCoV; ncov s1 Protein, 2019-nCoV; ncov s2 Protein, 2019-nCoV; ncov spike Protein, 2019-nCoV; NCP-CoV RBD Protein, 2019-nCoV; NCP-CoV s1 Protein, 2019-nCoV; NCP-CoV s2 Protein, 2019-nCoV; NCP-CoV Spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019-nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus s2 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; S2 Protein, 2019-nCoV; Spike RBD Protein, 2019-nCoV CoV spike 背景信息 The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. 研究領域 Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen 參考文獻 Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36. Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30. 相關推薦: CRISPR-Cas13a 重組CRISPR-Cas12a蛋白 Recombinant CRISPR-Cas12a protein 重組CRISPR-Cas9蛋白 Recombinant CRISPR-Cas9 protein SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) 重組TEV蛋白酶 Recombinant TEV Protease 重組SUMO蛋白酶 Recombinant SUMO Protease