SCI REP-UK ：山東農大丨NaCl脅迫下磁處理可調控楊樹N代謝

NMT是基因功能的活體檢測技術，已被103位諾貝爾獎得主所在單位，及北大、清華、中科院使用。

期刊：Scientific Report

主題：NaCl脅迫下磁處理可調控楊樹N代謝

標題：The effects of magnetic treatment on nitrogen absorption and distribution in seedlings of Populus?×?euramericana ‘Neva’ under NaCl stress

影響因子：4.011

檢測指標：NH4+、NO3-流速

檢測樣品：楊樹根伸長區（距根尖15mm）和葉肉細胞

NH4+、NO3-流實驗處理方法：

4周齡楊樹幼苗，分別經0g/L NaCl+磁化（M0），0g/L NaCl+非磁化（NM0），4g/L NaCl+磁化（M4），4g/L NaCl+非磁化（NM4）處理

NH4+、NO3-流實驗測試液成份：
NH4+: 0.1 mM NH4NO3, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5
NO3-: 0.1 mM NH4NO3, 1.0 mM KCl, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5

作者：山東農業大學林學院馬風云、劉秀梅

英文摘要

A potted experiment with Populus?×?euramericana ‘Neva’ was carried out to assess whether there are positive effects of magnetic treatment of saline water (MTSW) on nitrogen metabolism under controlled conditions in a greenhouse. Growth properties, nitrogen contents, enzyme activities and metabolite concentrations were determined based on field experiments and laboratory analysis after a 30-day treatment.

The results were as follows: (1) Biomass accumulation, root morphological properties and total nitrogen content were improved by MTSW. (2) Magnetization led to a greater increase in nitrate-nitrogen (NO3?-N) content in roots than in leaves, accompanied by greater NO3? efflux and activated nitrate reductase. (3) MTSW led to a higher ammonium-nitrogen (NH4+-N) content and greater uptake of net NH4+ in the leaves than that in the roots. (4) Magnetization stimulated glutamine synthase, glutamate dehydrogenase and glutamate synthase activities, whereas the concentrations of glutathione and oxidized glutathione were increased in leaves but decreased in roots, and the total glutathione content was increased.

Overall, these results indicated some beneficial impacts of MTSW on nitrogen translocation under field conditions, especially for equilibrating the distribution of NO3?-N and NH4+-N. Moreover, these findings confirmed the potential of using low-quality water for agriculture.

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中文摘要（谷歌機翻）

進行了盆栽的“ Neva”楊樹的盆栽實驗，以評估在受控條件下溫室中磁化鹽水（MTSW）對氮素代謝是否有積極作用。經過30天的處理后，根據田間實驗和實驗室分析確定了生長特性，氮含量，酶活性和代謝物濃度。

結果表明：（1）MTSW提高了生物量積累，根系形態特征和總氮含量。（2）磁化作用導致根部的**鹽氮（NO3-N）含量比葉片中的增加更大，同時伴隨著更大的NO3-流出量和活化的**還原酶。（3）MTSW導致葉片中的銨態氮（NH4+ -N）含量更高，并且比根部更高地吸收了凈NH4 +。（4）磁化刺激谷氨酰胺合酶，谷氨酸脫氫酶和谷氨酸合酶活性，而葉片中谷胱甘肽和氧化型谷胱甘肽的濃度增加，而根部減少，谷胱甘肽總含量增加。

總體而言，這些結果表明，MTSW對田間條件下的氮轉運有一些有益的影響，尤其是對于平衡NO3- -N和NH4+ -N的分布。此外，這些發現證實了將低質水用于農業的潛力。

結果表明：對于NO3-，葉肉細胞和根伸長區均表現為外排。存在NaCl脅迫時，磁療前后，葉肉細胞的NO3-外排速率從235.09 pmol·cm-2·s-1（NM4）增加到290.23 pmol·cm-2·s-1（M4）；經NaCl脅迫后，根伸長區的NO3-外排速率從123.53 pmol·cm-2·s-1（M4）增加到157.51 pmol·cm-2·s-1（NM4）。

至于NH4+，葉肉細胞表現為吸收而根伸長區表現為外排。經NaCl脅迫后，葉肉細胞的NH4+吸收速率降低，NM0和NM4并無顯著差異。但在根伸長區，NaCl脅迫后NH4+外排速率更高，M4的外排速率達到了186.83 pmol·cm-2·s-1。