NMT歷史上的今天丨AGR ECOSYST ENVIRON、Ecotoxicol Environ Saf 文章發表

NMT歷史上的今天

2018年10月22日，福建農林大學林文雄、林生利用NMT在Agriculture, Ecosystems and Environment上發表了標題為Rhizosphere responses to environmental conditions in Radix pseudostellariae under continuous monoculture regimes的研究成果。

2018年10月22日，華中農業大學孫學成、史凱麗利用NMT在Ecotoxicology and Environmental Safety 上發表了標題為Non-invasive microelectrode cadmium flux measurements reveal the decrease of cadmium uptake by zinc supply in pakchoi root (Brassica chinensis L.)的研究成果。

期刊：Agriculture, Ecosystems and Environment

主題：連續單培養條件下假星豆根際對環境條件的響應

標題：Rhizosphere responses to environmental conditions in Radix pseudostellariae under continuous monoculture regimes

影響因子：3.541

檢測指標：H+流速

作者：福建農林大學林文雄、林生

英文摘要

The root exudates of Radix pseudostellariae, working as a rhizospheric intermediary between plants and microbes, can deteriorate the microbial community in the rhizosphere in a consecutive monoculture system.

We assessed the e?ects of arti?cially applied R. pseudostellariae root exudates on R. pseudostellariae seedling growth, rhizo-sphere soil microbial communities, and soil physicochemical properties. We found that phenolic acids and or-ganic acids acted as a driver of changes in the microbial community. High-throughput sequencing and qRT-PCR analysis demonstrated that treatment with phenolic acids signi?cantly decreased the relative abundance of Trichoderma, Penicillium, Pseudomonadales, Xanthomonadales, and Streptomycetales.

Organic acids had a sig-ni?cant negative e?ect on the relative abundance of Pseudomonadales and Streptomycetales and signi?cantly increased the abundance of Fusarium, Xanthomonadales, Micrococcales, and Gemmatimonadales. Analysis based on the noninvasive microtest technique indicated that root exudates increased H+ e?ux and plasma membrane H+-ATPase activity in the pathogenic fungi and decreased them in the bene?cial fungi.

These phenomena created an acidic environment for the inhibition of bene?cial bacteria and accumulation of specialized plant pathogens. This study explains the mechanisms underlying the shift in micro?ora and structural disorder caused by root exudates in continuously monocultured R. pseudostellariae rhizosphere soil through responses to en-vironmental conditions.

中文摘要（谷歌機翻）

在植物和微生物之間作為根際中介體工作的偽星根的根系分泌物會在連續的單培養系統中破壞根際微生物群落。

我們評估了人工施用的擬星果根分泌物對擬星果幼苗生長，根際土壤微生物群落和土壤理化特性的影響。我們發現酚酸和有機酸是微生物群落變化的驅動力。高通量測序和qRT-PCR分析表明，用酚酸處理可顯著降低木霉菌，青霉菌，假單胞菌，黃單胞菌和鏈霉菌的相對豐度。

有機酸對假單胞菌和鏈霉菌的相對豐度有明顯的負面影響，并顯著增加了鐮刀菌，黃單胞菌，微球藻和Gemmatimonadales的豐度。基于非侵入性微測試技術的分析表明，根系分泌物增加了病原真菌中的H +流動和質膜H+ -ATPase活性，而減少了其有益真菌中的H+酶和質膜H+ -ATPase活性。

這些現象為抑制有益細菌和特殊植物病原體的積累創造了酸性環境。這項研究解釋了通過對環境條件的響應，在連續單培養的假星菌根際土壤中根系分泌物引起的微孔和結構紊亂轉變的潛在機制。

Fig. 7. E?ect of Fusarium oxysporum on steady H+ ?uxes in R. pseudostellariae roots. CK represents roots without F. oxysporum stimulation；F. oxysporum re-presents roots stimulated with F. oxysporum.

文章鏈接： https://linkinghub.elsevier.com/retrieve/pii/S0167880918304377

期刊：Ecotoxicology and Environmental Safety

主題：非侵入式微電極鎘通量測量顯示小菜根（甘藍）中鋅供應對鎘吸收的減少

標題：Non-invasive microelectrode cadmium flux measurements reveal the decrease of cadmium uptake by zinc supply in pakchoi root (Brassica chinensis L.)

影響因子：3.974

檢測指標：Cd2+流速

檢測部位：小白菜根部（距離靜止中心200um）

作者：華中農業大學孫學成、史凱麗

英文摘要

Zinc (Zn) possesses similar properties to cadmium (Cd) and inhibits Cd uptake in plants. To get more detailed mechanisms of Zn-inhibited Cd uptake in pakchoi, a hydroponic experiment was conducted to investigate the e?ects of various Zn levels on Cd concentrations, real time ?ux of Cd, expressions of genes related to Cd uptake under Cd exposure.

The results showed that the Cd concentrations and Cd accumulations in pakchoi root de-creased with increasing Zn levels, which were coincident with that real time Cd in?ux and net Cd in?ux of pakchoi root decreased with increasing Zn levels by non-invasive micro-test technology (NMT). Additionally, the expressions of Cd-related transporters including BcNRAMP5, BcIRT1 and BcMGT1 decreased with the increase of Zn levels under Cd exposure, especially BcIRT1 with the highest decreased rates. Furthermore, the expressions of these genes decreased gradually with the prolongation of Zn treated time under Cd toxicity.

The results indicate that Zn inhibits Cd uptake by inhibition of the expressions of Cd-related transporters, especially BcIRT1 in pakchoi root.

中文摘要（谷歌機翻）

鋅（Zn）具有與鎘（Cd）相似的特性，并抑制植物中Cd的吸收。為了更詳細地了解小白菜中Zn抑制Cd吸收的機理，進行了水培試驗，研究了各種Zn水平對Cd濃度，Cd實時通量以及Cd暴露下與Cd吸收有關的基因表達的影響。

結果表明，通過無創微試驗技術，小白菜根中的Cd濃度和Cd積累量隨Zn水平的升高而降低，這與小菜根的實時Cd輸入量和Cd凈含量隨Zn含量的增加而降低。 （NMT）。另外，隨著鎘暴露下鋅水平的升高，包括BcNRAMP5，BcIRT1和BcMGT1在內的Cd相關轉運蛋白的表達下降，尤其是下降率最高的BcIRT1。此外，隨著鎘對鎘的毒害作用時間的延長，這些基因的表達逐漸降低。

結果表明，Zn通過抑制小白菜根中Cd相關轉運蛋白特別是BcIRT1的表達來抑制Cd的吸收。

Fig. 3. E?ects of Zn on net Cd2+ in?uxes in root of pak-choi by Non-invasive micro-test technology. The 10 d pakchoi seedlings treated with Zn de?ciency Hoagland solution were used to analyze the Cd in?ux. The Zn con-centrations of 0 (Zn 0), 1 (Zn 1), 5 (Zn 5) and 10 μM (Zn 10) represented that the concentrations of Zn were added the basic solution containing 9 μM Cd. (A) The steady-state Cd2+ in?ux before and after the Zn supply into the basic solution. (B) Mean rates of Cd2+ in?ux of the time in A. Each point represents the mean of six roots from six in-dividual plants. Data are the means of 6 replicates ± standard error. Di?erent lowercase represents signi?cant di?erences between treatments (p < 0.05).

文章鏈接： https://doi.org/10.1016/j.ecoenv.2018.10.081