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化學生物學實驗技術>Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells
Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells
關鍵詞： monitoring spatiotemporal dynamics來源： 互聯網
Real-time and noninvasive imaging of intracellular second messengers in mammalian cells, while ?preserving their in vivo phenotype, requires biosensors of exquisite constitution. Here we provide the methodology for utilizing the single wavelength cGMP-biosensor δ-FlincG in aortic vascular smooth muscle cells.
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