熱門搜索詞：
Biotinylation?of?Antibody
關鍵詞： biotinylation antibody來源： 互聯網
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| Biotinylation of Antibody |
| Author: Nanci Donacki |
| Source: Contributed by Nanci Donacki |
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| Materials
Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room temperature or overnight at 4C. Dialyze against PBS. |
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