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  • 實驗方法> 免疫學技術> 抗體抗原實驗>骨組織的熒光染色

    骨組織的熒光染色

    關鍵詞: 骨組織 熒光 染色來源: 互聯網

    Fluorescent Label in Bone

    Some fluorescent compounds, which are fixed in newly formed calcified tissue, are used to label bone deposition. Such labels help to determine the time sequence of bone growth. The label may be given single or double label by intraperitoneal injection.

    Agents Sigma Cat # Color Dosage (mg/kg) Stock Con. (mg/ml) Diluents
    Calcein C-0875 Green 10 3 2%NaHCO3 PH7.4
    Xylenaol Orange(XO) X-0127 Red 90 30 2%NaHCO3 PH7.4
    Alizarin Complexone(AC) A-3882 Red 30 10 2%NaHCO3 PH7.4
    Oxytetracycline Hydrochloride(OH) O-5875 Yellow 30 10 20% EtOH

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    Age First Injection Before Sacrificing (days) Second Injection Before Sacrificing (days)
    <3 months 10 2
    >3 months, <6 months 15 5
    > 6 months 20 8

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