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Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
Human papillomaviruses (HPVs) are known to be etiological agents of cervical cancer and have been found in 99.7% of women with high-grade (HG) cervical intraepithelial neoplasia (CIN) precancer. Testing of high-risk HPV (HR-HPV) has been proposed as a way of improving cervical screening, especially for women with low-grade (LG) Papanicolaou (Pap) smears. In this chapter, real-time quantitative polymerase chain reaction (PCR) methods that can be used to investigate the expression of HPV 16 early genes in HG or LG precancer are demonstrated. Detecting the expression of early HPV genes in conjunction with the Pap smear may improve the specificity of identifying LG precancers that are associated with high risk of progression.
- 傳出神經藥理學實驗動物的選擇
- 國內外實驗動物科學發展情況
- Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic Raft Cultures
- Determining the Precursor Frequency of HBV Nucleocapsid Antigen-Specific T Cells
- Nucleotide Sequencing of Antigen Genes of Neisseria meningitidis
- Full-Length Infectious HCV Chimeras
- Bovine Spongiform Encephalopathy: Methods of Analyzing the Epidemic in the United Kingdom
- Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification
- Analysis of the Regulation of Viral Transcription
- Maintaining and Loading Neural Stem Cells for Delivery of Oncolytic Adenovirus to Brain Tumors
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