CD10 分子

CD10

常用單克隆抗體或代號：J5；(CALlA)

主要表達細胞：Pre-B，CAI。L，G[B]

分子質量(kDa)和結構：gp100(Ⅱ型膜分子)

功 能：為結合鋅的金屬蛋白酶，調節B細胞生長和增殖

CD10 Aka Common Acute Lymphoblastic Leukemia Antigen (CALLA), neutral endopeptidase 24.11, neprilysin, enkephalinase Cell membrane metallopeptidase, characteristic marker of follicular center cells and follicular lymphoma, but also widely distributed in normal tissue and neoplasms; also localized to brush border in small bowel mucosa

Inactivates bioactive peptides

Uses:

Acute lymphoblastic leukemia: one of first markers to identify leukemic cells in children (hence its name)

Breast: marker of myoepithelial cells, Mod Path 2002;15:397

Burkitt lymphoma: confirm diagnosis

Colonic carcinogenesis: increase in stromal cells from mild to severe dysplasia to invasive carcinoma, Hum Path 2002;33:806-8

Endometriosis: helpful in identifying areas of endometriosis if sparse glandular tissue

Follicular lymphoma: to confirm diagnosis

Hepatocellular carcinoma vs. nonhepatocellular carcinomas: 68% sensitive and >95% specific with canalicular pattern, AJSP 2001;25:1297, AJSP 2002;26:978, although another study recommends Hepatocyte, MOC31, and pCEA but not CD10, Mod Path 2002;15:1279

Microvillous inclusion disease: strong CD10+ cylasmic staining vs. linear brush border staining in normals, AJSP 2002;26:902

Positive staining (normal): adrenal cortex, pre-B cells, brain, choroid plexus, cortical thymocytes, endometrial stroma, follicular center cells, granulocytes, kidney microvilli, liver, lymphohemaoietic precursors, male GU epithelium, mesonephric remnants, myoepithelial cells (breast), neutrophils, ovary, placenta (cytotrophoblast, intermediate trophoblast, syncytiotrophoblast), small intestine (linear brush border staining)

Positive staining (disease): adenomyosis of endometrium, preB ALL (75%), CML in blast crisis (90%), colonic carcinoma, dermatofibroma, dermatofibrosarcoma, endometrial adenocarcinoma (may be present in desmoplastic stroma), endometrial stromal tumors, follicular center cell lymphomas, gastric carcinoma, glioma, hepatocellular carcinoma (canalicular pattern similar to polyclonal CEA), malignant mixed mullerian tumors, mediastinal germ cell tumors, melanomas, mesonephric tumors, microvillous inclusion disease (strong cylasmic staining), mullerian adenosarcoma, pancreatic adenocarcinoma, pancreatic solid-pseudopapillary tumor, placental site trophoblastic tumor, primary mediastinal B cell lymphomas (some), prostate carcinoma, renal cell carcinoma, renal cell sarcoma, rhabdomyosarcoma and other sarcomas, schwannoma, tumor of wolffian origin of broad ligament and ovary, urothelial carcinoma, uterine carcinoma, uterine cellular leiomyomas (50%), uterine leiomyosarcomas and other uterine sarcomas

Negative staining: myeloid and erythroid precursors, other female genital tract tumors (including clear cell carcinomas)

Micro images: follicular center cells (figure 1), intranodal heteroic mammary ducts (figure 1C),

Micro images (AJSP subscribers): endometrial stromal tumor #1 (negative), #2, hepatocellular carcinoma#1, #2, mesonephric carcinoma of uterus, mesonephric derivatives in men, microvillous inclusion disease#1, #2 (controls), vulvar, vaginal and cervical lesions, uterus and trophoblast, fallopian tube and ovary

Micro images (Hum Path subscribers): canalicular pattern in hepatocellular carcinoma (figure C), gastric carcinoma at apical border (figure B), colonic adenoma, colonic carcinoma#1, #2

Micro images (Mod Path subscribers): canalicular pattern in hepatocellular carcinoma (figure B), malignant mixed mullerian tumor#1, #2, #3, breast myoepithelium, breast adenosis, breast DCIS, invasive ductal carcinoma of breast, diffuse large B cell lymphoma, adenomyosis

References: AJSP 2002;26:978, AJSP 2001;25:1540, AJSP 2002;26:902, AJSP 2003;27:178, Mod Path 2002;15:1279, Mod Path 2002;15:923, Mod Path 2002;15:397, Mod Path 2002;15:413, Mod Path 2002;16:22