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mRNA展示技術
關鍵詞： mrna 展示 技術來源： 互聯網
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| 中文摘要 ： | ||
| ??????mRNA展示技術是一種新興的體外篩選多肽和蛋白質的有力工具. 在篩選過程中，mRNA與其編碼的多肽或蛋白質共價結合，形成mRNA-蛋白質融合體，能在大容量的多肽文庫 (1013 ～1015 ) 中篩選具有特定生物學功能的多肽和蛋白質. 目前，mRNA展示技術主要應用于各種靶分子的多肽和蛋白質適體的發現以及蛋白質相互作用機制的闡明和分析. 由于其自身的巨大發展潛力，mRNA展示技術具有更為廣闊的應用前景. | ||
| 英文摘要 ： | ||
| ??????mRNA display provides a new powerful tool for in vitro selecting of peptides and proteins. In the selecting process, peptides are covalently attached to their own mRNA to form mRNA-protein fusions. These mRNA-protein fusions enable in vitro selection of peptide and protein libraries of more than 1013 different sequences. The experiment conditions and protocols have been optimized in recent years. The application of mRNA display technology is mainly in the discovery of ligands for many kinds of target molecules and the analysis and mechanism elucidation on interaction between proteins. With its great potential, there will be a wide application foreground in the application of mRNA display. | ||
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推薦方法
- MicroRNA?and?siRNA?Cloning?Protocol
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