RNA Extraction by the Proteinase K Method
關鍵詞: rna extraction proteinase來源: 互聯網
This method of RNA extraction relies on a relatively gentle lysis procedure that should burst the cells, but leave the nuclei intact. Contamination of a relatively RNase-free cytoplasmic environment with nuclear nucleases is thus minimised. Next the polysomes are dissociated with SDS and proteinase K and finally the protein is removed by several phenol/choloroform extractions (1 ,2 ).
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