Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System
To date, the cDNAs and genes encoding three distinct forms of the enzyme nitric oxide synthase (NOS) have been described (reviewed in ref. 1 ). The family of NOS enzymes generate nitric oxide (NO) from arginine, producing citrulline as a co-product and requires the presence of heme, tetrahydrobiopterin (BH4 ), nicotinamide adenine dinucleotide phosphate (NADPH), calmodulin (CaM), flavin mononucleotide (FMN), and flavine adenine nucleotide (FAD) (2 ). The NO generated by the family of NO synthases has been found to play an important role in many biological processes involved in health and disease including neurotransmission, the mediation of vasodilation, and host defense (2 ).
- RNA Oligonucleotide Synthesis Via 5-Silyl-2-Orthoester Chemistry
- Characterization of Interactions Between Proteins Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectros
- Amicon?Stirred?Ultrafiltration?Cells
- Analysis of Cellular Migration Using a Two-Chamber Methodology
- In SituHybridization
- X-Ray Scattering and Solid-State Deuterium Nuclear Magnetic Resonance Probes of Structural Fluctuations in Lipid Membranes
- 層析分離技術發展歷程
- 酸堿滴定法
- 人抗磷脂酶A2抗體(Anti-PLA2R)酶聯免疫分析(ELISA)
- Analysis of Phosphatidylinositol 3,4,5 Trisphosphate 5-Phosphatase Activity by in vitro and in vivo Assays