Tecan 推出具有實時細胞成像的 Spark 細胞板讀取器

30 6月 2019

SLAS Europe 2019為Tecan提供了展示其Spark Cyto板讀取器的跳板，提供生物、化學和物理事件的實時檢測和分析。

基于原始Spark平臺的成功，新產品將高端多模讀板儀的靈活性與全孔成像和基于細胞的測定的全面環境控制相結合。

Spark Cyto使用頂級相機組件和正在申請專利的LED自動對焦系統，為6至384孔格式提供實時數據采集和分析，確保不會錯過任何關鍵事件。它允許將定性和定量信息集成到多參數數據集中，從而比以往更快地提供有意義的見解。憑借三個放大倍率水平和四個采集通道，只需一張圖像即可捕獲96孔或384孔微孔板的整個孔面積，從而可以研究整個細胞群，而不會出現平鋪或失真。

SparkControl 和圖像分析儀軟件使操作員能夠完全控制所有實驗參數，并為常見細胞術應用提供預定義的方法。結合用戶可編程的高級功能，例如用于自動執行動力學實驗的實時實驗控制，它旨在為基于細胞的研究開辟新的可能性。

Nerviano Medical Sciences細胞分析主管，Spark Cyto的早期用戶Fabio Gasparri博士說：“該系統的自動細胞孵化和分析功能給我留下了深刻的印象。與專用的細胞培養箱方法相比，細胞以類似的倍增時間生長，甚至更加均勻。96孔板的全孔成像能力允許實時進行可重復的分析。

奧地利格拉茨大學分子藥學教授Valery Bochkov博士對此表示贊同：“使用Spark Cyto進行細胞成像，酶測試和免疫測定，我們對該軟件的直觀性和易用性印象深刻。我們的文憑和博士生立即學會了如何使用Spark Cyto并獲得高質量的結果。

Tecan unveils Spark Cyto plate reader with real-time image cytometry

30 Jun 2019

SLAS Europe 2019 provided the springboard for Tecan to showcase its Spark Cyto plate reader – offering real-time detection and analysis of biological, chemical and physical events.

Building on the success of the original Spark platform, the new product combines the flexibility of a high-end multimode plate reader with whole well imaging and comprehensive environmental control for cell-based assays.

Spark Cyto uses top-of-the-range camera components and a patent-pending LED autofocus system to provide real-time data acquisition and analysis – for 6- to 384-well formats – ensuring that no key event is missed. It allows qualitative and quantitative information to be integrated into multiparametric data sets, delivering meaningful insights faster than ever before. With three magnification levels and four acquisition channels, it enables entire cell populations to be investigated by capturing the whole well area of 96- or 384-well microplates in just one image, without tiling or distortion.

SparkControl and Image Analyzer software gives the operator complete control of all experimental parameters, with predefined methods for common cytometry applications. Together with user-programmable advanced features – such as Real Time Experimental Control for automatic performance of kinetic experiments – it is designed to unlock new possibilities for cell-based research.

Dr Fabio Gasparri, Head of Cell Assays at Nerviano Medical Sciences and an early user of Spark Cyto, said: “I was impressed by the system’s automated cell incubation and analysis capabilities. Cells were growing with a similar doubling times, and even more homogeneously, compared to a dedicated cell incubator approach. The whole well imaging capability for 96-well plates allowed reproducible analysis in real time.”

Dr Valery Bochkov, Professor of Molecular Pharmaceutics at the University of Graz, Austria, agreed: “Using the Spark Cyto for cell imaging, enzymatic tests and immunoassays, we were impressed by how intuitive and easy to use the software is. Our diploma and PhD students instantly learned how to work with Spark Cyto and obtain high quality results.”